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Titlebook: Methods of Protein Microcharacterization; A Practical Handbook John E. Shively Book 1986 Springer Science+Business Media LLC 1986 amino aci

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COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methodsvolume), this cannot be achieved by Edman degradation because this stepwise procedure does not allow discrimination between polypeptides with free or blocked COOH-terminal carboxylic groups. This shortcoming of Edman degradation must not be ignored in view of the significant biologic role of COOH-te
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nsights into the structure-function of neurotransmitter receptors and ion channels in the brain. These techniques involve immunoreactions with antibodies before embedding and ultrathin sectioning. As a result, they are very sensitive and show excellent resolution and good ultrastructural and antigen
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發(fā)表于 2025-3-29 14:56:12 | 只看該作者
nsights into the structure-function of neurotransmitter receptors and ion channels in the brain. These techniques involve immunoreactions with antibodies before embedding and ultrathin sectioning. As a result, they are very sensitive and show excellent resolution and good ultrastructural and antigen
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Petro E. Petrides without compromising the integrity of antibody-binding sites by tissue fixation, which is required for conventional immunocytochemistry. Fixation introduces covalent modifications, cross-linking and/or denaturation of proteins. These chemical modifications often alter the antibody-binding sites, an
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John E. Shivelyins. For this purpose, expression vectors based on Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus, all members of the alphavirus genus, have been engineered in the form of naked RNA replicons, recombinant viral particles, and layered DNA vectors. By applying these alph
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