| 書目名稱 | Methods of Preparation for Electron Microscopy | | 副標(biāo)題 | An Introduction for | | 編輯 | David G. Robinson,Ulrich Ehlers,Friedrich-Wilhelm | | 視頻video | http://file.papertrans.cn/633/632412/632412.mp4 | | 圖書封面 |  | | 描述 | In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes- sions had an unexpected instrument at their disposal which exceeded the reso- lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob- lems inherent in specimen preparation for the investigation of cellular struc- tures. The microtechniques applied in light microscopy were no longer appli- cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada- tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses- sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolutio | | 出版日期 | Book 1987 | | 關(guān)鍵詞 | Thin section; antibody; cell; cell culture; electron microscopy; electron optics; microscopy; nucleic acid; | | 版次 | 1 | | doi | https://doi.org/10.1007/978-3-642-48848-1 | | isbn_softcover | 978-3-540-17592-6 | | isbn_ebook | 978-3-642-48848-1 | | copyright | Springer-Verlag Berlin Heidelberg 1987 |
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