Titlebook: Methods of Hybridoma Formation; Arie H. Bartal,Yashar Hirshaut Book 1987 The Humana Press Inc. 1987 DNA.Expression.Interferon.Macrophages.

Graduated

Mechanisms of Cell Fusion and Selection in the Generation of Hybridomas,actually the culmination of advances in several unrelated fields of research. In this review the various cell fusion and selection techniques that allowed for the development of the hybridoma technology will be considered. In addition, some recent advances in cell hybridization and selection procedu

GRIPE

Enhancement of Hybridoma Formation,rt, who reported in 1912 the formation of large polykaryons from cultured macrophages treated with lycopodium spores (1). In subsequent years, the appearance of multinucleated cells following spontaneous in vitro cell fusion was sporadically described (2–4), all involving cells of the same lineage.

Cardiac-Output

Modification of HAT Medium and Hybridoma Formation,ion. HAT medium originally developed by Littlefield in 1964 (1) has been one of the key factors that has made hybridoma generation practical. The value of this medium is generally considered from the viewpoint of its ability to inhibit unfused myeloma cell proliferation. We have recently explored mo

懦夫

魯莽

Nucleate

lethal

UTTER

In Vitro Immunization for the Generation of Hybridomas Using Serum-Free Medium,ecognize specific sites on a single antigen and often bind to these sites with high affinity. Because of this, researchers have found many uses for monoclonal antibodies, including their application in immunoassays (1), purification or identification of cellular proteins (2,3), and studies of enzyme

兩種語(yǔ)言

Use of Heteromyelomas in the Enhancement of Human Monoclonal Antibody Production,t of the murine hybridoma technology (1). At the present time it is routine to generate murine monoclonal antibodies of defined specificity by fusion of mouse myeloma cells with spleen cells from an immunized mouse. The ready availability of murine monoclonal antibodies as biological probes has chan

arousal

Human Hybridomas, murine hybridoma Mabs has been refined enormously since it was introduced (2), the technology for generating human Mabs has until now lagged behind, chiefly because of the paucity of suitable human cell lines that can serve as fusion partners and support the secretion of immunoglobulin (Ig). Hypoxa