Titlebook: Methods in Protein Structure Analysis; M. Zouhair Atassi,Ettore Appella Book 1995 Springer Science+Business Media New York 1995 Amino acid

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態(tài)度暖昧

Robert L. Moritz,James Eddes,Richard J. Simpson to reveal its intrinsic properties by using nonparametric methods. This volume contains a selection of contributions presented at the XVIII Annual Congress of the Portuguese Statistical Society..978-3-642-42972-9978-3-642-32419-2Series ISSN 2194-7767 Series E-ISSN 2194-7775

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nstrate that the entire family is a flexible device that adapts to many interesting situations of modern scientific practice where the number of observations stays fixed or grows very slowly while the number of automatically measured features grows dramatically and only a small fraction of these fea

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instate

High-Speed Chromatographic Separation of Proteins and Peptides for High Sensitivity Microsequence Anfluoride (Fernandez et al., 1992) and nitrocellulose (Aebersold et al., 1987), following electrotransfer from the gel. Protein identification can be achieved by microsequence analysis of the isolated peptides using either automated Edman degradation or tandem mass spectrometry (Hunt et al., 1986; Bu

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Synthesis, Evaluation and Application of a Panel of Novel Reagents for Stepwise Degradation of Polyp et al, 1990, 1991; Aebersold et al, 1991; Gooley et al, 1991; Pisano et al, 1993), modified and unnatural amino acids of known structure are difficult to identify and de-novo characterization of such residues by UV absorbance detection alone is extremely difficult.

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Deacetylation and Internal Cleavage of Polypeptides for N-Terminal Sequence Analysision, long handling times and inaccessibility of the N-terminal fragment to Edman degradation, are avoided. The protocol has been applied to both a synthetic peptide corresponding to the N-terminal segment of horse liver alcohol dehydrogenase and to the intact protein.