Titlebook: Methods for studying the genetics, molecular biology, physiology, and pathogenesis of the streptococ; Paula M. Fives-Taylor,Donald J. LeBl

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Site-specific homologous recombination mutagenesis in group B streptococci,uences into a temperature sensitive plasmid and selecting for clones which have undergone homologous recombination. The recombinan clones can be easily isolated by selecting for clones which have retained the antibiotic resistance markers that are present on the vector or cloned into the gene-specif

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A lactococcal pWV01-based integration toolbox for bacteria, of plasmids that are all based on the broad-host-range lactococcal replicon pWV01 which has been deprived of its gene encoding the replication initiation protein RepA. These so-called pORI plasmids can only replicate if RepA is provided .. Special . and . helper strains, producing RepA . (RepA.), a

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Characterization of the lactococcal conjugative element pRS01 using IS,-mediated mutagenesis,development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS.-mediated cointegration with the lacto. shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions inc

Allodynia

Use of electroporation in genetic analysis of enterococcal virulence,cies. We demonstrate the use and the advantages of this technique for investigating genes, both chromosomal and plasmid-linked, encoding surface structures. Electroporation was used to deliver constructs created on shuttle vectors for insertional inactivation of a chromosomal gene involved in bindin

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