Titlebook: Methods for Protein Analysis; A Practical Guide fo Robert A. Copeland Book 1994 Springer Science+Business Media Dordrecht 1994 amino acid.C

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Introduction to Protein Structure,nctions. Proteins provide the structural rigidity . flexibility associated with external body parts such as skin, hair, and the exoskeletons of arthropods. By changing their structure they provide the basis for biological motions, such as muscle contraction. A large class of proteins, the enzymes, s

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Immunological Methods,ty of a protein, and to screen samples (e.g., column fractions) for the presence of a specific protein. Two of the most common immunochemical methods for protein analysis are Western blots and antibody-capture assays. These shall be described in detail in this chapter. Descriptions of other immuno-c

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Peptide Mapping and Amino Acid Analysis,ng the sequential arrangement of amino acids in a protein or peptide has been in place for some time. The first protein for which a full amino acid sequence was determined was the peptide hormone insulin by Frederick Sanger in 1953. At the time, this work was considered a major breakthrough, since i

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Protein Folding and Stability, the polypeptide chain. This correctly folded structure, referred to as the native conformation, is thought to represent not only the most biologically active form of the protein, but also an arrangement of the polypeptide chain of minimal potential energy. While it is true that there is some thermo