Titlebook: Methods for Fragments Screening Using Surface Plasmon Resonance; Sameer Mahmood Zaheer,Ramachandraiah Gosu Book 2021 The Editor(s) (if app

構(gòu)想

https://doi.org/10.1007/978-981-16-1536-8Surface plasmon resonance; Protein stability; Fragment library; Thermal shift assay; Amine coupling

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Preparation of Protein with AviTag? for Biotin-Based Capturelecules by avidin–biotin-based methods is very common due to the robustness of the interaction. Preparation of AviTag? protein for SPR is the primary work to initiate the binding studies. This chapter will outline the preparation of AviTag? protein.

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Sameer Mahmood Zaheer,Ramachandraiah GosuDiscusses real-time and label-free measurement of biomolecular interaction.Present protocols to calculate kinetics and affinities with single analyte concentration.Examines techniques for the optimum

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Screening of Fragments in SPRHigh throughput screening using the SPR system has revolutionized drug discovery in identifying hits. Several SPR platforms are available with their respective software to setup the screening cascade. We used Pioneer software to input various parameters required for a successful screening.

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Selection and Identification of Fragment Libraryplications of criteria that in general result in the selection of a diverse set of fragments keeping in mind their later expansion into full small molecules and their development into possible drugs with good profiles.