Titlebook: Metagenomics; Methods and Protocol Wolfgang R. Streit,Rolf Daniel Book 2023Latest edition The Editor(s) (if applicable) and The Author(s),

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CRISPR-Cas9 Shaped Viral Metagenomes Associated with ,RISPR-Cas9 system with an artificial CRISPR-array can be set up and used to discriminate abundant and well-known . phage from a host-based metagenome enrichment. The obtained viral suspension can be used for metagenome sequencing and isolating new viral strains.

干涉

declamation

The PET-Degrading Potential of Global Metagenomes: From In Silico Mining to Active Enzymes,o screening method to find new PETases in metagenomic datasets. This method can easily be adapted to find other enzyme classes. We also list a number of assays that can be used to test the enzymes for activity on PET as well as other substrates.

nonchalance

反叛者

Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Un gene has increased during the last years, the vast majority of marine diversity are rather unexplored. Moreover, most studies focused on the entire microbial community and thus do not assess the active fraction of the microbial community. Here, we describe a detailed protocol for the simultaneous e

Cardiac

Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compouvironment. Consequently, the marine environment is an exceptional depot of novel bioactive natural products, with structural and chemical features generally not found in terrestrial habitats. Here, in particular, microbes represent a vast source of unknown and probably new physiological characterist

Commentary

Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resisur ability to prepare for, and respond to, emerging AMR threats is dependent on our knowledge of genes able to confer AMR that are circulating within various environmental, animal, and human microbiomes. Targeted, sequence-specific, detection of AMR genes and functional resistance assays, described

強(qiáng)所

Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next-Generation Sequencing Ay those encoded by polyketide synthase (PKS) clusters due to their astounding chemical diversity. While most discovered compounds originate from culturable microorganisms, yet-to-be cultured microbes represent a reservoir of previously inaccessible compounds. The advent and development of metagenomi

震驚

Manifest

Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity,line phosphatases, are recognized as useful biocatalysts in applications such as plant and animal nutrition, bioremediation, and diagnostic analysis. Here, we describe a functional metagenomics approach enabling rapid identification of genes encoding these enzymes. The target genes are detected base