Titlebook: Metabolic Profiling; Methods and Protocol Thomas O. Metz Book 2011 Springer Science+Business Media, LLC 2011 Drug analysis.Global metabolom

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節(jié)省

Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism,ly in blood and urine. For years, the most widespread technique in use was ion-exchange chromatography followed by post-column derivatization with ninhydrin, a method which is the basis of numerous automated AA analyzers with a throughput of about eight samples/day. The emergence of tandem mass spec

減至最低

Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry, of metabolic disorder accumulate disease-specific acylcarnitines that correlate with the acyl coenzyme A compounds in the affected mitochondrial metabolic pathways. For example, propionylcarnitine accumulates in patients with both propionic and methylmalonic acidemias. The test identifies and quant

Instrumental

tooth-decay

,HPLC Analysis for the Clinical–Biochemical Diagnosis of Inborn Errors of Metabolism of Purines and ates that induce transient or permanent alterations of metabolism. In particular, the diagnosis of several inborn errors of metabolism (IEMs) is accomplished by the analysis of circulating and excreted purines and pyrimidines. It is certainly advantageous to simultaneously determine the full purine

MOT

Bile Acid Analysis in Various Biological Samples Using Ultra Performance Liquid Chromatography/Elecd exact BA behavior, the precise distribution of BAs in vivo must be determined. However, to date, it has been difficult to know the precise roles of BA due to variations in BA molecules including conjugated forms. Thus, we reconsidered BA extraction methodology and introduced an ultra performance l

acolyte

Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrome gas chromatography (GC)-MS as complementary methods. With IC-MS-MS, pyruvate, glucose-6-phosphate, fructuse-6-phosphate, fructose-1,6-bisphosphate, phosphoenolpyruvate, and the sum of 2-phosphoglyceraldehyde + 3-phosphoglyceraldehyde can be quantified. With GC-MS using selected ion monitoring, glyc

BET

Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry,ntly related and unrelated pathways of metabolism. Relationships of the CAC intermediates, as measured by their concentrations and relative ratios, offer useful information to understanding interrelationships between the CAC and metabolic pathways under various physiological and pathological conditi

Cardiac

countenance

High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)-Based Drug Metabolite Profiling,overy and development. Mass spectrometry, particularly when combined with high-performance liquid chromatography (HPLC-MS), can enable detailed structural information to be obtained on the metabolites of a drug or xenobiotic as a result of metabolism. The successful identification of drug metabolite