Titlebook: Membrane Trafficking; Methods and Protocol Jingshi Shen Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

Hemochromatosis

書目名稱Membrane Trafficking影響因子(影響力)




書目名稱Membrane Trafficking影響因子(影響力)學(xué)科排名




書目名稱Membrane Trafficking網(wǎng)絡(luò)公開(kāi)度




書目名稱Membrane Trafficking網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書目名稱Membrane Trafficking被引頻次




書目名稱Membrane Trafficking被引頻次學(xué)科排名




書目名稱Membrane Trafficking年度引用




書目名稱Membrane Trafficking年度引用學(xué)科排名




書目名稱Membrane Trafficking讀者反饋




書目名稱Membrane Trafficking讀者反饋學(xué)科排名

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Detonate

Membrane Trafficking978-1-0716-2209-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

reserve

Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp TechniqueWhole-cell patch clamping is a standard method to monitor the secretion of synaptic vesicles. In this chapter, we describe the basic steps of whole-cell patch clamping for measuring synaptic exocytosis, aiming to provide reference for researchers who are new to this field.

SOBER

https://doi.org/10.1007/978-1-0716-2209-4Endomembrane systems; Exocytic pathway; Endocytic pathway; Membrane biology; CRISPR genetic screens

collagen

Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook scently tagged cargo of interest is retained in the endoplasmic reticulum and released in a biotin-dependent manner. Here, we report a detailed protocol describing the steps necessary to perform RUSH experiments to study secretory granule biogenesis at the trans-Golgi network (TGN).

craven

In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusionoteins. For example, synip and tomosyn negatively regulate GLUT4 SNARE-mediated membrane fusion. Here we describe in vitro reconstituted assays to determine the molecular mechanisms of SNAREs, synip, and tomosyn. These methods can also be extended to the studies of other types of membrane fusion events.

xanthelasma

Imaging Single-Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)ws for visualization of membrane dynamics at the cell surface with superb spatiotemporal resolution. In this chapter, TIRFM is used to record and analyze exocytosis of single glucose transporter-4 (GLUT4) containing vesicles in 3T3-L1 adipocytes.

賠償

Julia Dancourt,Grégory Lavieuwith detailed examples and exercises at the end of each chap.Inventories are prevalent everywhere in the commercial world, whether it be in retail stores, manufacturing facilities, government stockpile material, Federal Reserve banks, or even your own household. This textbook examines basic mathemat