Titlebook: Meiosis; Methods and Protocol Jesús A Carballo Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spring

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Book 2024s through methods on genomics, biochemistry, super-resolution microscopy, traditional genetics, cytological methods, as well as machine learning and .in silico. modelling. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respectiv

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brachial-plexus

Efficient Enrichment of Synchronized Mouse Spermatocytes Suitable for Genome-Wide Analysisies we adapted for rapid and reliable isolation of synchronized fixed mouse spermatocytes. We show that chromatin isolated from these cells can be used to study chromatin-binding sites by ChIP-seq. High-quality data we obtained from INO80 ChIP-seq in zygotene cells was used for functional analysis of chromatin-binding sites.

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ellagic-acid

SPO-Seq: An Accessible Method for Efficient Evaluation of Spo11 Catalytic Activity and Profiling Mei early meiosis. This involves controlled double-strand breaks (DSBs) formation catalyzed by Spo11. DSBs exhibit a preferential location in specific genomic regions referred to as hotspots, and their variability is tied to varying Spo11 activity levels. We have refined a ChIP-Seq technique, called SP

Surgeon

Hi-C2B: Optimized Detection of Chromosomal Contacts Within Synchronized Meiotic , Cellsal pairwise interactions into counts of pairwise interactions. To study the many temporally regulated facets of meiotic recombination in ., the Hi-C assay must be robust such that fine- and wide-scale comparisons between genetic datasets can be made. Here we describe an updated protocol for Hi-C (Hi

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Efficient Enrichment of Synchronized Mouse Spermatocytes Suitable for Genome-Wide Analysis controls fundamental meiotic processes such as recombination and homologous chromosome associations. Recent game-changing advances have been made by analysis of chromatin binding sites of meiotic specific proteins genome-wide in mouse spermatocytes. However, further progress is still highly depende

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A Method to Study the Meiotic Recombination Map in Human Preimplantation Blastocystsnt populations. However, only few gametes from individual could?generate offspring, which limits its exploration in nature selection. In the last few years, preimplantation blastocysts?based on trio SNP-chip data were available in individuals for preimplantation genetic testing (PGT). In this protoc

針葉樹

Generation of Meiotic Mouse Models Using CRISPR/Cas9 Technologythe molecular mechanisms governing a myriad of biological processes. Within this scientific landscape, the investigation of meiosis in mice has attracted considerable attention across numerous research laboratories. The precision and versatility of the CRISPR/Cas9 genome editing system have revoluti

Inoperable

Isolation of Homogeneous Sub-populations of Spermatocytes from Mouse Testisar mechanisms governing meiosis remain largely unknown, primarily due to the difficulty in isolating pure sub-populations of spermatocytes. Definitive molecular, biochemical, and functional investigations of the meiosis process require the isolation of these individual homogeneous sub-populations of