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Titlebook: Meiosis; David T. Stuart Book 2017Latest edition Springer Science+Business Media New York 2017 Gametogenesis.Budding yeast models.Computat

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發(fā)表于 2025-3-21 19:55:01 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Meiosis
編輯David T. Stuart
視頻videohttp://file.papertrans.cn/631/630256/630256.mp4
概述Includes cutting-edge methods and protocols for meiosis research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Meiosis;  David T. Stuart Book 2017Latest edition Springer Science+Business Media New York 2017 Gametogenesis.Budding yeast models.Computat
描述This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful?.Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Detailedand practical, .Meiosis, Second Edition. will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation..
出版日期Book 2017Latest edition
關(guān)鍵詞Gametogenesis; Budding yeast models; Computational methods; Chromosome reorganization; Gene expression n
版次2
doihttps://doi.org/10.1007/978-1-4939-6340-9
isbn_softcover978-1-4939-8169-4
isbn_ebook978-1-4939-6340-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

書目名稱Meiosis影響因子(影響力)




書目名稱Meiosis影響因子(影響力)學(xué)科排名




書目名稱Meiosis網(wǎng)絡(luò)公開度




書目名稱Meiosis網(wǎng)絡(luò)公開度學(xué)科排名




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書目名稱Meiosis被引頻次學(xué)科排名




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沙發(fā)
發(fā)表于 2025-3-21 23:36:04 | 只看該作者
Book 2017Latest editionl methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies
板凳
發(fā)表于 2025-3-22 03:29:02 | 只看該作者
Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in ,,nation and chromosome segregation. Here we describe genetic methods for analysis progression of . through meiosis and sporulation with an emphasis on strategies for the genetic analysis of regulators of meiosis-specific genes.
地板
發(fā)表于 2025-3-22 08:02:23 | 只看該作者
Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy,ytes in detail as they undergo maturation. Our method is based on tracking the “center of brightness” of fluorescently labeled chromosomes. Here we describe how to set up our software and run experiments on a Leica TCS SP8 confocal microscope, but the method would be transferable to other microscopes with computer-aided microscopy.
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發(fā)表于 2025-3-22 17:40:35 | 只看該作者
Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast,tions of DNA breaks to the base pair. In this chapter we detail the steps involved in Spo11-oligo mapping in the SK1 strain of budding yeast ., from harvesting cells of highly synchronous meiotic cultures, through preparation of sequencing libraries, to the mapping pipeline used for processing the data.
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Fluorescent Protein as a Tool for Investigating Meiotic Recombination in ,,nalysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for .. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.
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發(fā)表于 2025-3-23 05:45:10 | 只看該作者
In Vivo Imaging of Budding Yeast Meiosis,is method allows tracking of individual cells over extended periods of time through every stage of the meiotic transformation while minimizing phototoxicity and sustaining conditions that support meiotic growth.
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