Titlebook: Mechanobiology; Methods and Protocol Ronen Zaidel-Bar Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity Sensing byrecise control of the geometry of growth, and standardization of the measurements. In sum, MLS enables one to quantitatively test, even on long time scales, the effect of the rigidity and the geometry of the environment on the growth of roots and root hair cells, including mechanotransduction to the nucleus.

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Quantifying Strain-Sensing Protein Recruitment During Stress Fiber Repairthen either be compared to (Blanchoin, Physiol Rev 94, 235–263, 2014) a known control (e.g., zyxin-GFP) or (Hoffman, Mol Biol Cell 23, 1846–1859, 2012) the pre-damaged stress fiber protein distribution. With this method, strain-sensing proteins and their dynamic association with stress fiber strain sites can be reproducibly measured and compared.

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Photoresponsive Hydrogels for Studying Mechanotransduction of Cellss incorporation into a polyacrylamide hydrogel to render it photoresponsive. Precise control over the physical properties of the gel allows observation of glioblastoma durotaxis under surface stiffness conditions relevant to the actual brain environment.

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Quantification of Invadopodia Formation and Matrix Degradation Activitygrading proteinases, and apply protrusive forces generated by the actin cytoskeleton, which drive the invasive process. Here, we describe a fluorescent microscopy-based protocol for imaging and quantifying both invadopodia formation and matrix degradation.

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Imaging Cell Adhesive Force at the Single Molecule Levely. ITS can be selectively implemented at two imaging modes: a cumulative mode that maps cell adhesive force at a high signal-to-noise ratio even with a low-end fluorescence microscope, and a real-time mode that images the force at the single molecular tension level.

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1064-3745 expertsThis detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features m

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Measuring Cellular Traction Forces with Micropillar Arrays of cell spreading on the pillars. This technique offers the advantage of high spatial and temporal resolution analyses and constitutes a method to investigate the effect of substrate rigidities on cellular functions.

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