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Titlebook: Measuring Calcium and Calmodulin Inside and Outside Cells; Ole H. Petersen Book 2001 Springer-Verlag Berlin Heidelberg 2001 Calcium.Fluore

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書目名稱Measuring Calcium and Calmodulin Inside and Outside Cells
編輯Ole H. Petersen
視頻videohttp://file.papertrans.cn/629/628170/628170.mp4
概述Step by step protocols of the various techniques for measuring Ca2+ inside and outside cells and organelles.Protocols contain the experience of researchers who did pioneering work in the field of meas
叢書名稱Springer Lab Manuals
圖書封面Titlebook: Measuring Calcium and Calmodulin Inside and Outside Cells;  Ole H. Petersen Book 2001 Springer-Verlag Berlin Heidelberg 2001 Calcium.Fluore
描述The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signalling molecules as well as very many pathophysiological processes including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles and in the immediate surroundings of individual cells are described in practical details by experts, on the basis of their personal experience. Considering that the protein calmodulin is the main target for Ca2+ action in most cells types, a chapter on tracking calmodulin inside cells, using fluorescence technology, has also been included.
出版日期Book 2001
關(guān)鍵詞Calcium; Fluorescence measurements; calcium distribution; calmodulin; cancer; cell; cells; electrophysiolog
版次1
doihttps://doi.org/10.1007/978-3-642-56851-0
isbn_softcover978-3-642-47737-9
isbn_ebook978-3-642-56851-0
copyrightSpringer-Verlag Berlin Heidelberg 2001
The information of publication is updating

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Measuring Ca2+ in the Nucleoplasm of Intact Cellsien (Tsien et al. 1982; Grynkiewicz et al., 1985), is that the protein does not permeate across cell membranes and thus needs to be microinjected. The technique was thus limited to large, sessile cells. The isolation of the aequorin cDNA (Inouye et al., 1985) completely changed this perspective. Ind
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their personal experience. Considering that the protein calmodulin is the main target for Ca2+ action in most cells types, a chapter on tracking calmodulin inside cells, using fluorescence technology, has also been included.978-3-642-47737-9978-3-642-56851-0
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Measuring Calcium and Calmodulin Inside and Outside Cells
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refines the approximate solution; and the final stage applies a partial lexicographic search procedure to generate an optimal solution. Extensive computational experiments show that our algorithms are able to solve large problems of size up to one million variables in less than 7 seconds CPU time on
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