Titlebook: Matrix Metalloproteases; Methods and Protocol Charles A. Galea Book 2017 Springer Science+Business Media LLC 2017 matrix metalloprotease.X-

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Heterologous Expression of the Astacin Protease Meprin β in ,ered a preferable expression system. Here, we describe the manipulation and secretory expression of human meprin β in the methylotrophic yeast .. We show that the position of the affinity tag strongly influences the yield of expression, favoring fusion of the affinity tag at the C-terminus.

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Structural Studies of Matrix Metalloproteinase by X-Ray Diffraction inclusion bodies using a refolding protocol that yielded significant quantities of active protein. Crystals of MT1-MMP were obtained using the vapour diffusion method. Here, we describe the protocols used for crystallization and the data analysis together with the resulting diffraction pattern.

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Krishna K. Singh,Ruchi Jain,Harini Ramanan,Deepak K. Sainithe society, especially those of heavy-rainfall events due to their high potential to cause hazards like flooding, landslide, and debris flow. During the past decade, heavy-rainfall QPFs have been shown to improve considerably by using a cloud-resolving model at a grid size of 2.5?km in Taiwan, wher

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