Titlebook: Maternal Placental Interface; Methods and Protocol Verónica Zaga-Clavellina Book 2024 The Editor(s) (if applicable) and The Author(s), unde

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Evaluation of Leukocyte Chemotaxis Induced by Human Fetal Membranes in an In Vitro Model,preterm labor with or without infection. During pregnancy, the fetal membranes act as a physical barrier that isolates the fetus into the amniotic cavity, keeping it in an optimal environment for its development. In addition, the fetal membranes possess immunological competencies such as the secreti

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In Vitro Culturing of Human Trophoblasts from Term Placenta,g their ability to differentiate into syncytiotrophoblasts and secrete steroid and peptide hormones that help sustain a viable pregnancy. This task was addressed by testing different serum concentrations, cell culture media, digestive enzymes, growth factors, substrate coating with diverse proteins

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Culture of Human Fetal Membranes in a Two Independent Compartment Model: An Ex Vivo Approach,ernal and fetal. These tissues have the function of delimiting the amniotic cavity. Their histological complexity gives them physical, mechanical, and immunological properties to protect the fetus. Although the study of the amnion, chorion, and decidua separately provides knowledge about the functio

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CRISPR Activation in Mouse Trophoblast Stem Cells,st lineage and mesodermal derivatives, which merge through the chorioallantoic fusion process in a critical event for the progression of placenta development. The trophoblast lineage is derived from self-renewing, multipotent cells known as mouse trophoblast stem cells (mTSCs). These cells are a val

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Feto-Maternal Interface Organ-on-Chip: A New Technology to Study Ascending Infection,adverse pregnancy outcomes, the two feto-maternal interfaces (FMi) that form during gestation (i.e., the placenta and fetal membrane) need to be investigated to understand their biological roles, and organ dysfunction can lead to adverse outcomes. Adverse pregnancy outcomes such as preterm rupture o

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Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-maternal Interfacmune cells from the feto-maternal interface. However, difficulty and inconsistency in sampling approaches of immune cells and short lifespan in vitro prevent their usage in mechanistic studies to understand the maternal-fetal immunobiology. To address these limitations, existing cell line models can