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Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Arnaud Delobel Book 2021 Springer Science+Business Media, LLC, part of Springer Na

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發(fā)表于 2025-3-21 16:25:14 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Mass Spectrometry of Glycoproteins
副標(biāo)題Methods and Protocol
編輯Arnaud Delobel
視頻videohttp://file.papertrans.cn/626/625276/625276.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Mass Spectrometry of Glycoproteins; Methods and Protocol Arnaud Delobel Book 2021 Springer Science+Business Media, LLC, part of Springer Na
描述This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Mass Spectrometry of Glycoproteins: Methods and Protocols. is a valuable resource for scientists interested in learning more about this
出版日期Book 2021
關(guān)鍵詞glycosylation; HILIC-MS; Bioinformatics; MALDI-MS; N-glycans
版次1
doihttps://doi.org/10.1007/978-1-0716-1241-5
isbn_softcover978-1-0716-1243-9
isbn_ebook978-1-0716-1241-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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發(fā)表于 2025-3-21 22:24:26 | 只看該作者
Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Massr the reader a practical overview of the available methodologies for the confident analysis of intact glycoproteins with different glycosylation degree by MALDI-TOF MS. The three proposed methods fulfil the requirements of reproducibility and low extent of glycan fragmentation required to successfully analyze intact glycoproteins.
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發(fā)表于 2025-3-22 06:35:03 | 只看該作者
Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS,mide bonded stationary phases packed with sub-2?μm particles has allowed for remarkable separations also at the subunit level. Here, we describe a simple protocol to perform the mAb glycans profiling at subunit level by HILIC/MS.
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發(fā)表于 2025-3-22 09:23:28 | 只看該作者
,Site-Specific O-Glycosylation Analysis by Liquid Chromatography–Mass Spectrometry with Electron-Tranalysis during the development of therapeutic glycoproteins. Here, we describe a method of in-depth site-specific O-glycosylation analysis by liquid chromatography–mass spectrometry using electron-transfer/higher-energy collisional dissociation (EThcD) and database analysis.
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發(fā)表于 2025-3-22 16:04:45 | 只看該作者
Routine Analysis of ,-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection,l-enhancement label, LC-MS data generation, and data analysis. Using this method, up to 24 .-glycan samples can be prepared at one time and analyzed by LC-MS. With the addition of automation platform, up to 96 .-glycan samples can be prepared and analyzed in a high-throughput manner.
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