Titlebook: Mass Spectrometry in Biology & Medicine; A. L. Burlingame,Steven A. Carr,Michael A. Baldwin Book 2000 Springer Science+Business Media New

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Electron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and , n from a single ECD spectrum of multiply protonated ubiquitin (76 amino acids) provides, ., ~90% of its sequence, and ECD of smaller proteins and peptides generally provides 90%–100% of the sequence. However, this coverage decreases for proteins >10 kDa, and proteins >20 kDa only undergo multiple re

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Deciphering Functionally Important Multiprotein Complexes by Mass Spectrometry,vides clues to understanding in molecular detail how cellular activities are carried out. Dissection of the protein complex places its individual subunits in a context of the specific cellular function carried out by the entire assembly. Taken together with specific motifs recognized in the sequence

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Investigation of Apoptosis-Involved Processes by Mass Spectrometric Identification of the Apoptosiss of membrane integrity. In contrast, apoptotic cell death is characterized by controlled autodigestion of functional, important proteins of the cell connected with cellular shrinkage, membrane blebbing and chromatin condensation. The nuclear DNA of apoptotic cells is often fragmented. The membrane

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