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Titlebook: Mass Spectrometry Data Analysis in Proteomics; Rune Matthiesen Book 2007 Humana Press 2007 Mascot.Proteomics.Translation.algorithms.bioinf

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發(fā)表于 2025-3-21 19:38:24 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Mass Spectrometry Data Analysis in Proteomics
編輯Rune Matthiesen
視頻videohttp://file.papertrans.cn/626/625255/625255.mp4
概述Essential reference for mass spectrometry (MS) in proteomics and glycomics.Concise protocols for optimizing search parameters in MS search engines.Practical guide to MS data analysis and bioinformatic
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Mass Spectrometry Data Analysis in Proteomics; Rune Matthiesen Book 2007 Humana Press 2007 Mascot.Proteomics.Translation.algorithms.bioinf
描述Mass Spectrometry Data Analysis in Proteomics is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. As MS is a high throughput technique, proteomic researchers must attend carefully to the associated field of data analysis, and this volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters. Several different types of MS data are discussed, followed by a description of optimal methods for conversion of raw data into peak lists for input to search engines. Choosing the most accurate and complete databases is emphasized, and a report of available sequence databases is included. Methods for assembling expressed sequence tags (ESTs) into assembled nonredundant databases are provided, along with protocols for further processing the sequences into a format suitable for MS data. Mass Spectrometry Data Analysis in Proteomics describes publicly available applications whenever possible.
出版日期Book 2007
關(guān)鍵詞Mascot; Proteomics; Translation; algorithms; bioinformatics; databases; sequence analysis
版次1
doihttps://doi.org/10.1385/1597452750
isbn_softcover978-1-61737-644-3
isbn_ebook978-1-59745-275-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
The information of publication is updating

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Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintiotential internal calibrants, like tryptic autodigest peptides and keratin-related peptides, can be identified and used for high-precision calibration. Furthermore, the construction of project/user-specific lists of potential calibrants is illustrated.
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Quantitative Proteomics by Stable Isotope Labeling and Mass Spectrometry, several methods for the introduction of mass tags to proteins and peptides for MS-based quantitative proteomic analysis, including isotope-coded affinity tags, stable isotope labeling by amino acids in cell culture, global internal standard technology, and mass-coded abundance tagging.
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Useful Mass Spectrometry Programs Freely Available on the Internet,o a limited extend been tested. The links provided in this chapter will over time become invalid. In such cases it is worth while to try a World Wide Web search using the program packages names. This will often reveal the updated links. Some of the links presented in this chapter will also be maintained at ..
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Extracting Monoisotopic Single-Charge Peaks From Liquid Chromatography-Electrospray Ionization-Masseaks can subsequentially be exported to other programs or used internally by VEMS to perform peptide mass fingerprinting searches or peptide quantitation. Additionally, VEMS interfaces the commercial program ProteinLynx Global server v2.0.5 for automatic peak extraction from MS/MS spectra obtained by LC-MS/MS.
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