Titlebook: Mammalian Synthetic Promoters; David Gould Book 2017 Springer Science+Business Media LLC 2017 synthetic promoters.gene therapy.pharmacolog

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書目名稱Mammalian Synthetic Promoters影響因子(影響力)




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書目名稱Mammalian Synthetic Promoters被引頻次學(xué)科排名




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書目名稱Mammalian Synthetic Promoters讀者反饋學(xué)科排名

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1064-3745 n advice from the experts.This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful .Methods in Molecular Biology .series

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Monitoring Promoter Activity by Flow Cytometryow cytometry and represents a readout for the promoter activities. In this chapter, the protocol for measurement and analyzing of transfected cells expressing the reporter gene EGFP is thoroughly described and fully illustrated.

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Initial Considerations Before Designing a Promoter Construct,or perhaps even to function in vivo? The final application will have a bearing on promoter design and vector of choice from the start of the study. In this chapter I highlight some of the vector attributes to consider and features that should be thought about.

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Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assayalso to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demonstrate interactions of endogenous transcription factors with sequences derived from endogenous and synthetic promoters.

FAZE

Secreted Reporters for Monitoring Multiple Promoter Function applications and limitations for monitoring of promoter function. We will also describe both cell-based and blood-based assays for detecting three commonly used reporters: secreted alkaline phosphatase (SEAP), . luciferase (Gluc), and . luciferase (Vluc).

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