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Titlebook: Mammalian Cell Engineering; Methods and Protocol Ryosuke Kojima Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 202

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發(fā)表于 2025-3-21 18:26:46 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Mammalian Cell Engineering
副標(biāo)題Methods and Protocol
編輯Ryosuke Kojima
視頻videohttp://file.papertrans.cn/623/622033/622033.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: Mammalian Cell Engineering; Methods and Protocol Ryosuke Kojima Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 202
描述This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooti
出版日期Book 2021
關(guān)鍵詞dCas9-based chimeric receptors; SynNotch; SUPRA-CAR; Riboswitches; CRISPR
版次1
doihttps://doi.org/10.1007/978-1-0716-1441-9
isbn_softcover978-1-0716-1443-3
isbn_ebook978-1-0716-1441-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

書(shū)目名稱Mammalian Cell Engineering影響因子(影響力)




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Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cellsration of synthetic receptors in unexplored contexts is cumbersome, especially for nonspecialist laboratories. Here, I provide a detailed protocol on how to use receptors of the generalized extracellular molecule sensor (GEMS) platform. GEMS is a modular receptor system that can be adapted to sense
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Engineering Mammalian Cells to Control Glucose Homeostasiseutic strategies allowing well-controlled production and release of insulinogenic hormones into the blood circulation are required. In this chapter, we describe how mammalian cells can be engineered for applications in diabetes treatment. While closed-loop control systems provide automated and self-
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Sonogenetic Modulation of Cellular Activities in Mammalian Cellsbeen widely used for clinical diagnosis for several decades. Ultrasound induces bioeffects in tissues, providing potential value in therapeutic applications. However, the intrinsic millimeter scale of the ultrasound focal zone represents a challenge with respect to minimizing the illuminated regions
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Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diamedicine. Using a multidisciplinary design principle coupling electrical engineering, software development, synthetic biology, and optogenetics, the investigators developed a smartphone-controlled semiautomatic theranostic system that regulates blood glucose homeostasis in diabetic mice in an ultrar
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Construction of Caffeine-Inducible Gene Switches in Mammalian Cellsies, inducers of gene expression which demonstrate the highest degree of safety and patient adherence are needed. In this chapter, I describe methods to implement caffeine-controlled gene expression systems into mammalian cells. Using an array of different implementation strategies, from reconstitut
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