Titlebook: Macromolecular Drug Delivery; Methods and Protocol Mattias Belting Book 2009 Humana Press 2009 Antigen.DNA.In vitro delivery.In vivo delive

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Ludwig G. Strauss,Antonia Dimitrakopoulou-Straussay or in order to create links between the past and the present (Said, 2000, p. 179). Post-communist countries in Eastern Europe also faced this task, and they tried to solve the problem through various means, among others numerous vehicles of memorialization. Such efforts included the renaming of p

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1064-3745 face between disciplines. Ultimately, this cooperation will lead to the use of macromolecular drugs as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat di978-1-62703-966-6978-1-59745-429-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Poly(,-amino esters): Procedures for Synthesis and Gene Delivery,for synthesis of these polymers, particle self-assembly, and transfection using these particles are delineated. A standard protocol is presented as well as a high-throughput screening technique that can be used to more quickly optimize transfection parameters for efficient delivery.

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siRNA and DNA Transfer to Cultured Cells,d. Hence, this chapter attempts to explain some of the popular cationic lipid/polymer-based transfection reagents for . DNA/small inhibitory RNA (siRNA) delivery, mainly focusing on the protocols and critical factors to keep in mind to ensure successful delivery of nucleic acids into eukaryotic cells using these methods.

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