Titlebook: Lymphoma; Methods and Protocol Ralf Küppers Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2019 Lase

滴注

RNA Sequencing in B-Cell Lymphomas,NA (cDNA) to generate several millions of short sequence reads that are aligned to a reference genome allowing the comprehensive characterization of the transcripts in a cell. RNA-Seq has a wide variety of applications which lead to a pervasive adoption of this method well beyond the genomics commun

悠然

Studying Cancer Heterogeneity by Single-Cell RNA Sequencing,e. Genetic and epigenetic changes in combination with external cues and selective forces are the driving factors behind tumor heterogeneity. Understanding this variability within and across patients may partly explain the unpredictable outcomes of cancer treatments. Measuring the variation of gene e

瑪瑙

Evaluation of Whole Genome Sequencing Data, analysis of WGS data, including alignment, identification of different classes of genomic variants, the identification of driver mutations, and the identification of mutational signatures. We further outline design considerations for WGS studies and provide a variety of quality control measures to

哺乳動物

Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines,the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells during the screen, isolate genomi

我怕被刺穿

Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens,ion of biomarkers associated with response to drugs would greatly improve the efficacy of drug treatment. Platforms that can comprehensively map drug response in high-throughput ex vivo provide a unique tool to identify associated biomarkers and provide hypotheses for mechanisms underlying variable

hedonic

Proximity Ligation Assay, technique used to examine protein-protein interactions in situ. PLA offers many advantages over traditional biochemical approaches to signaling in that it can detect both transient interactions, which are hard to capture otherwise, as well as protein interactions within large supramolecular signali

樂意

正論

Ralf KüppersIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

關心

Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/589223.jpg

變化無常

Springer Science+Business Media, LLC, part of Springer Nature 2019