Titlebook: Lung Innate Immunity and Inflammation; Methods and Protocol Scott Alper,William J. Janssen Book 2018 Springer Science+Business Media, LLC,

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Isolation and Characterization of Human Alveolar Type II CellsIsolation of human ATII cells provides a valuable tool to study their function under normal and pathophysiological conditions. Moreover, maintenance of their differentiated phenotype in vitro allows further study of their function. Here we describe a protocol for efficient ATII cell isolation, characterization, and culture.

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Isolation and Characterization of Human Lung Myeloid Cellstype, and function. Here we describe an approach to process lung tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This base antibody panel can also be used to identify major immune cell types in human blood and bronchoalveolar lavage (BAL) fluid.

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Measuring Neutrophil Bactericidal Activity. Regardless of the mechanism, the ability to kill pathogens is a vital outcome. This chapter describes a method to measure the in vitro bactericidal activity of isolated neutrophils as the endpoint of converging innate immune functions.

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Computational Analysis of RNA-Seq Data from Airway Epithelial Cells for Studying Lung Diseasesoftware packages currently available. The aim of this chapter is to provide a broad overview of the major steps involved in processing and analyzing RNA-seq data, with a special focus on methods optimized for data generated from AECs. We take the reader from the point of obtaining sequence reads fr

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Paleontology

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