Titlebook: Lotus japonicus Handbook; Antonio J. Márquez Book 2005 Springer Science+Business Media B.V. 2005 Assimilat.DNA.Expression.RNA.enzymes.gene

ALIEN

精確

Mutagenesis gamma (γ-) irradiation and chemical mutagenesis. Advantages and disadvantages of these two methods are presented, along with an outline of procedures for and indicators of successful mutagenesis in .. Screening procedures for symbiotic mutants, tannins and starch mutants are also described.

CANT

神秘

A procedure for , nodulation studiesenes or the phenotypes of wild type and mutant lines. We present here a feasible, reliable procedure for . nodulation studies of .. All the different steps of the symbiotic interaction have been analysed and different parameters of plant growth followed to demonstrate the reproducibility of the procedure.

厚顏無恥

Embedding root and nodule tissue in plastic (BMM). We have used plastic embedded tissue from . roots and young nodules successfully in immunolocalisation experiments and developed a protocol that can also be adapted for . RNA localisation studies. The different parameters tested are described, as well as the use of alkaline phosphatase- or fluorescently-conjugated secondary antibodies.

考得

RNA isolation using CsCl gradientsnly contaminated with genomic DNA, proteins, and polysaccharides. Here we describe a RNA extraction method that yields high quality RNA from different tissue types of Lotus japonicus (Sambrook et al. 1989).

garrulous

殺死

Arbuscular mycorrhizac techniques for rapid and consistent AM infection and for the visualisation of AM structures in plant roots. These techniques are useful for molecular and cellular studies and have been adapted to the specific requirements of ..

menopause

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