Titlebook: Listeria monocytogenes; Methods and Protocol Kieran Jordan,Edward M. Fox,Martin Wagner Book 2014 Springer Science+Business Media New York 2

明顯

書目名稱Listeria monocytogenes影響因子(影響力)




書目名稱Listeria monocytogenes影響因子(影響力)學科排名




書目名稱Listeria monocytogenes網(wǎng)絡公開度




書目名稱Listeria monocytogenes網(wǎng)絡公開度學科排名




書目名稱Listeria monocytogenes被引頻次




書目名稱Listeria monocytogenes被引頻次學科排名




書目名稱Listeria monocytogenes年度引用




書目名稱Listeria monocytogenes年度引用學科排名




書目名稱Listeria monocytogenes讀者反饋




書目名稱Listeria monocytogenes讀者反饋學科排名

性冷淡

Multilocus Sequence Typing (MLST) of ,r methods as it can reconstruct ancestral and evolutionary linkage between . isolates. MLST detects all genetic variations within the amplified housekeeping gene that accumulate slowly. This chapter describes how to undertake MLST.

明智的人

Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of ,ated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype . strains.

Ardent

fulmination

口訣法

Generation of Nonpolar Deletion Mutants in , Using the “SOEing” Method. For mutagenesis in ., we describe two different plasmid-based approaches, which facilitate the introduction of this spliced amplicon in place of the corresponding segment of chromosomal DNA: the pKSV7 system and the pORI280/pVE6007 system.

LIKEN

Sampling the Processing Environment for ,to sample, the best time for sampling and the frequency of sampling are also given. Such details help hygienists/microbiologists to be successful in their attempts to isolate strains of ., even if such bacteria are well attached to surfaces or located in niches that are difficult to reach.

ANA

含鐵

ROOF

Extraction and Analysis of Plasmid DNA from ,ient for analytical purposes but may be upscaled for further downstream analysis. Electrophoretic separation of the resultant lysate allows conclusions to be made on the presence, number, copy number, and size of the plasmids in the analyzed bacterial strains.