Titlebook: Liposomes; Methods and Protocol Gerard G.M. D‘Souza,Hongwei Zhang Book 2023Latest edition The Editor(s) (if applicable) and The Author(s),

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From Olive Oil Emulsions to COVID-19 Vaccines: Liposomes Came First,llectual curiosity, which began with sprinkling olive oil on seawater and culminated in playing around with smears of egg lecithin on microscopic slides. This brief review highlights how a few pairs of gifted hands attached to highly motivated brains have turned a curious discovery made under a micr

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Preparation of DRV Liposomes, of hydrophilic solutes, compared to other passive-loading liposome preparation methods. These characteristics make this liposome type ideal for entrapment of labile substances, such as peptides, proteins, or DNA’s (or other nucleotides or oligonucleotides), or in general biopharmaceuticals and sens

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Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation,aking a thin lipid film in a round-bottom flask by the removal of organic solvent. Upon the addition and agitation of the dispersion medium, heterogeneous liposomes are formed. Finally, after extrusion through polycarbonate membranes, homogeneous small liposomes are obtained.

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Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing,e nanoliposome synthesis over a wide range of production rates can be readily achieved using a high aspect ratio flow focusing device fabricated by widely available additive manufacturing methods. Here we detail methods for the manufacture and operation of a 3D-printed microfluidic flow focusing tec