Titlebook: Liposomes; Methods and Protocol Gerard G.M. D‘Souza Book 2017Latest edition Springer Science+Business Media New York 2017 pharmacology.memb

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Determination of the Subcellular Distribution of Liposomes Using Confocal Microscopy,s technique suitable for such studies. In this chapter, we describe the application of confocal laser scanning microscopy (CLSM) to determine the subcellular distribution of fluorescently labeled mitochondriotropic liposomes.

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Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes,e at pH 5.0 to 5.5 can be easily prepared by inserting a hydrophobically modified .-isopropylacrylamide/methacrylic acid copolymer (poly(NIPAM-.-MAA)) in the lipid bilayer of sterically stabilized liposomes. The present chapter describes polymer synthesis, as well as the preparation and characterization of large unilamellar pH-sensitive vesicles.

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Book 2017Latest editiond avoiding known pitfalls..Comprehensive and practical, .Liposomes: Methods and Protocols, Second Edition. is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry..

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Liposomes Came First: The Early History of Liposomology,ered by intellectual curiosity, which began with sprinkling olive oil on seawater and culminated in playing around with smears of egg lecithin on microscopic slides. This brief review highlights how a few pairs of gifted hands attached to highly motivated brains have launched Liposome Technology.

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Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation,aking a thin lipid film in a round-bottom flask by the removal of organic solvent. Upon the addition and agitation of the dispersion medium, heterogeneous liposomes are formed. Finally, after extrusion through polycarbonate membranes, homogeneous small liposomes are obtained.