Titlebook: Lipidomics; Paul Wood Book 2017 Springer Science+Business Media LLC 2017 Lipids.Mass Spectrometry Imaging.Oxysterols.Plasmenyl Glycerophos

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Introduction and Overview of Lipidomic Strategies,ganism. The metabolome can thus be regarded as closer to the actual phenotype than either the genome or proteome. Lipids represent a major component of the metabolome and their hydrophobic and amphipathic nature dictates their separate analysis from more water-soluble metabolites; hence, the discipl

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Nontargeted Lipidomics Utilizing Constant Infusion High-Resolution ESI-Mass Spectrometry,lipid subfamilies utilizing a 1 min constant infusion. This approach allows broad sampling of the lipidome while minimizing issues of lipid losses, of racemization with silica-based supports, and of ghost effects that are associated with almost all chromatographic systems for lipids containing very-

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Ultrahigh-Resolution Lipid Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometrse. Integrated omics is defined as the correlation of interdisciplinary technologies to interpret the molecular interactions within a biological organism, in response to gene/chemotherapy or disease state, by monitoring and correlating: phenomics, genomics, transcriptomics, lipidomics, proteomics, P

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MALDI-Imaging Mass Spectrometry of Brain Lipids,ne such biomolecule, lipids, is an important, but often times challenging species to analyze. Here, we describe the matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) of lipids and glycolipids from mammalian brain in an untargeted and high-throughput approach.

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Ion-Mobility Mass Spectrometry for Lipidomics Applications,etry, is a promising new tool for supporting lipidomics research. IM is a gas-phase electrophoretic technique that enables the separation of ions in the gas phase according to their charge, shape, and size. IM separation, which occurs on a time scale of milliseconds, is compatible with modern mass s

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In Vitro Assay to Extract Specific Lipid Types from Phospholipid Membranes Using Lipid-Transfer Procking in eukaryotic cells: vesicular and non-vesicular. Lipid-transfer proteins (LTPs) that catalyze the inter-membrane transfer of lipids play pivotal roles in non-vesicular systems. As a key biochemical feature, LTPs extract a specific lipid type from membranes in vitro. This chapter describes two

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Mass Spectrometric Determination of Fatty Aldehydes Exemplified by Monitoring the Oxidative Degradaes, given its high selectivity and sensitivity. However, the analysis of fatty aldehydes, which are important components of cell metabolism, remains challenging. Usually, chemical derivatization prior to MS detection is required to enhance ionization efficiency. In this regard, the coupling of fatty