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Titlebook: Lipid Signaling Protocols; Mark G. Waugh Book 2016Latest edition Springer Science+Business Media New York 2016 cell function.ceramides.cho

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發(fā)表于 2025-3-21 16:24:15 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Lipid Signaling Protocols
編輯Mark G. Waugh
視頻videohttp://file.papertrans.cn/587/586856/586856.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Lipid Signaling Protocols;  Mark G. Waugh Book 2016Latest edition Springer Science+Business Media New York 2016 cell function.ceramides.cho
描述.In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. .Lipid Signaling Protocols, Second Edition. guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Lipid Signaling Protocols, Second Edition. aims to ensure successful results in the further study of this vital field..?.
出版日期Book 2016Latest edition
關(guān)鍵詞cell function; ceramides; cholesterol-rich lipid rafts; detergent-resistant membrane; lipid signaling; me
版次2
doihttps://doi.org/10.1007/978-1-4939-3170-5
isbn_softcover978-1-4939-5007-2
isbn_ebook978-1-4939-3170-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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發(fā)表于 2025-3-21 20:14:35 | 只看該作者
,Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative Hilinositol-5-phosphate 4-kinase α (PI5P4Kα) utilizing the .-myo-di16-PtIns(5)P substrate in quantitative high-throughput screening (qHTS) format using the ADP-Glo? technology to couple the production of ADP to a bioluminescent readout.
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地板
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Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-Kinasly identified actin cytoskeleton regulators—the WASH complex and RhoGEF1, as major novel interactors of PI4KIIα. While this chapter focuses on the PI4KIIα interactome, this protocol can and has been used to generate other membrane interactome networks.
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,High-Throughput Fluorometric Assay for Membrane–Protein Interaction, rapid, sensitive, and accurate determination of lipid specificity and affinity for various lipid binding domains and proteins, and also high-throughput screening of small molecules that modulate membrane binding of proteins.
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發(fā)表于 2025-3-22 18:08:24 | 只看該作者
1064-3745 ation advice from the experts.In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism
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發(fā)表于 2025-3-22 23:47:52 | 只看該作者
Book 2016Latest editions techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. .Lipid Signaling Protocols, Second Edition. guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the t
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發(fā)表于 2025-3-23 03:35:55 | 只看該作者
Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity,ll as for the identification of their ligands. In this chapter we describe in detail the assays performed to investigate LXR activity in a macrophage-like cell line (RAW 267.4). These can be easily adapted to other nuclear receptors and transcription factors.
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發(fā)表于 2025-3-23 08:40:44 | 只看該作者
,Using Surface Plasmon Resonance to Quantitatively Assess Lipid–Protein Interactions,ar interactions and the kinetics and affinity of an interaction. This technique has been especially useful in measuring the affinities of lipid-binding proteins to intact liposomes of varying lipid compositions.
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