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Titlebook: Lipase and Phospholipase Protocols; Mark Doolittle,Karen Reue Book 1999 Humana Press 1999

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發(fā)表于 2025-3-21 20:04:08 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Lipase and Phospholipase Protocols
編輯Mark Doolittle,Karen Reue
視頻videohttp://file.papertrans.cn/587/586829/586829.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Lipase and Phospholipase Protocols;  Mark Doolittle,Karen Reue Book 1999 Humana Press 1999
描述The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman‘s disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from an
出版日期Book 1999
版次1
doihttps://doi.org/10.1385/1592595812
isbn_softcover978-1-61737-069-4
isbn_ebook978-1-59259-581-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1999
The information of publication is updating

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發(fā)表于 2025-3-21 22:50:43 | 只看該作者
1064-3745 tous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to con
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發(fā)表于 2025-3-22 03:02:12 | 只看該作者
Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Akage of plasmalogen may also play an important role in protecting cellular membranes against oxidative stresses (.,.). The breakdown of plasmalogen may be a receptor-mediated process related to signal transduction (.,.).
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發(fā)表于 2025-3-22 04:45:01 | 只看該作者
Bile Salt-Activated LipaseAL activity are of interest because they reflect various aspects of enzyme function, such as the regulation of the enzyme activity by bile salt and by its substrates. In this chapter, we summarize the procedure for the assay, purification and expression of human BAL in ..
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Lecithin-Cholesterol Acyltransferasel processes is supported by the finding of plasma and tissue accumulation of free cholesterol and the presence of nascent, discoidal HDL particles in the plasma of familial LCAT-deficient subjects (.).
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Assays for Pancreatic Triglyceride Lipase and Colipaseaboratory. The remainder of the Introduction will discuss methods utilized in both settings, and then two assays suitable primarily for the research laboratory are described in . and ., including the assay of colipase in ..
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發(fā)表于 2025-3-23 08:12:45 | 只看該作者
Purification of Lipases and Phospholipases by Heparin-Sepharose Chromatographyn to the equilibrating buffer or by using substances such as salts or denaturants (.). Theoretically, the success of heparin-Sepharose chromatography depends largely upon how closely the conditions used in the experiment mimic the native biological interactions.
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