Titlebook: Leishmania; Methods and Protocol Joachim Clos Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Neglected Tropic

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,Syrian Hamster as an?Advanced Experimental Model for Visceral ,s with . species causing visceral leishmaniasis (VL) closely mimic the disease in the natural hosts, including target organs, lesions, and clinical course. Therefore, despite some shortcomings (e.g., genetic background, price, and scarcity of reagents), it is probably the best laboratory rodent mode

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Experimental Cutaneous ,: Mouse Models for Resolution of Inflammation Versus Chronicity of Disease,parasite control and resolution of inflammation as well as of parasite evasion and chronicity of disease. In addition, over many years .infected mice have been successfully used to analyze the function of newly discovered immune cell types, transcription factors, cytokines, and effector mechanisms i

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LeishGEdit: A Method for Rapid Gene Knockout and Tagging Using CRISPR-Cas9,ifferent tags to generate N- or C-terminally tagged proteins. We describe how to use an online platform (.) for automated primer design and how to perform PCRs and transfections in small batches or on 96-well plates for large-scale knockout or tagging screens. This method allows generation of knocko

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1064-3745 luable guide for both established and novice researchers in the .Leishmania. field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide.?.978-1-4939-9210-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Dan Zilberstein,Roni Nitzan Korenbbau der St?rpotentiale, setzt eine genaue "Lokalisierung" der Probleme voraus, denn eine "schnelle Probleml?sung" erzeugt neue Probleme. Von daher wird von B. Bitzer erst eine umfassende Erfassung geleistet und dann werden einige M?glichkeiten zur Redu- zierung der dargestellten Innovationshemmnisse angespro978-3-663-01666-3978-3-663-01665-6