Titlebook: Legume Genomics; Methods and Protocol Mukesh Jain,Rohini Garg Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020

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Methods of Gene Expression Profiling to Understand Abiotic Stress Perception and Response in Legumeitions. In order to achieve food and nutritional security, legume breeding programs should also incorporate advanced genomics tools. Genomes of many model and nonmodel legume crops have been sequenced, which provide opportunities to identify and characterize candidate genes to develop abiotic stress

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Method for Bisulfite Sequencing Data Analysis for Whole-Genome Level DNA Methylation Detection in L DNA methylation is inherited from one generation to another leaving an epigenetic mark as a memory of previous state, which may include encounter with stress or pathogen. Advancement in the next generation sequencing technologies has enabled the profiling of methylation marks. Whole-genome bisulfit

BRIEF

Method for Genome-Wide Association Study: A Soybean Example,est. There are now a variety of R-packages and command line tools available to perform GWAS. Here, we provide an example downloading and filtering SNP data, followed by GWAS analysis using the R-package rMVP.

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Genotyping for Species Identification and Diversity Assessment Using Double-Digest Restriction Siteods to generate a reduced representation of the genome for sequencing, GBS can provide genome-wide single nucleotide polymorphisms (SNP) for diversity analysis at high throughput and low cost. Here we describe a novel double-digest restriction site-associated DNA sequencing (ddRAD-seq) approach. We

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LAITY

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Machine Learning-Based Annotation of Long Noncoding RNAs Using PLncPRO,ross diverse species, including plants and animals, and are known to be involved in regulation of various biological processes. To understand their biological significance, we first need to identify the lncRNAs accurately. However, distinguishing lncRNAs from coding transcripts is still a challengin

ensemble

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Method for Characterization of Root Traits in Chickpea Germplasm for Legume Genomics and Breeding,large set of chickpea (.) germplasm. The system was designed to accommodate a large number of plants in a small area allowing relatively deeper root development, and thus serves as a high-throughput phenotyping tool for studying root dynamic growth. The root trait quantitative platform could provide

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Methods for Screening Legume Crops for Abiotic Stress Tolerance through Physiological and Biochemicer, different genotypes may exhibit significant variations between individual responses, which can determine their tolerance or susceptibility to these stresses. The present chapter suggests a broad range of assays that can help in understanding stress perception by plants at cellular and molecular