Titlebook: Kinesin Protocols; Isabelle Vernos Book 2001 Humana Press 2001

與生

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書目名稱Kinesin Protocols讀者反饋




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Kernel

Isabelle VernosIncludes supplementary material:

PHON

使聲音降低

Purification of Kinesin from the Brain,The kinesin superfamily constitutes a structurally diverse group of microtubule-based motor proteins that use the energy of Mg-ATP hydrolysis to transport organelles along microtubules (.). Purification of kinesins is required for both the characterization of their motile properties and studies of their regulation.

defuse

Plasmids for Expression of Chimeric and Truncated Kinesin Proteins,The construction of mutant or chimeric proteins enables researchers to identify regions of a protein that are involved in a particular function. The technique of overlap polymerase chain reaction (PCR) (.) allows one to make a number of mutants in a timely fashion.

Chemotherapy

遺留之物

變態(tài)

虛構(gòu)的東西

The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindlghly dynamic bipolar structure assembles and disassembles periodically and its main structural components are microtubules (MTs) and chromosomes. Microtubule-dependent motors in particular members of the kinesin superfamily play an important role in directing the self-organization of microtubules into a bipolar spindle.

Relinquish

Identification of Kinesin-Associated Proteins,re hypothesized to provide regulation of the vesicle-binding and the ATPase activities of the kinesin heavy chain, effectively regulating kinesin-mediated vesicle motility. Thus, the identification of these kinesin-associated proteins would greatly increase our understanding of how kinesin function is regulated in vivo.