Titlebook: Kinase Screening and Profiling; Methods and Protocol Hicham Zegzouti,Said A. Goueli Book 2016 Springer Science+Business Media New York 2016

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Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy,lar interaction between the protein and the ligand, and has many advantages in fragment screening over other biophysical techniques. This chapter describes the practical aspects of fragment screening by saturation transfer difference NMR.

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Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells,visualizing the protein phosphorylation in living cells. Using these indicators, we visualized under a fluorescence microscope when, where, and how the protein kinases are activated in single living cells.

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周年紀(jì)念日

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Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Formacan be coupled to FLuc through a two-step process. This chapter describes the bioluminescent assays that were developed for three classes of kinases (lipid, protein, and metabolic kinases) and miniaturized to 1536-well format, enabling their use for quantitative high-throughput (qHTS) of small-molecule libraries.

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A High-Content Assay to Screen for Modulators of EGFR Function,on are imaged and quantified using time-dependent granule formation in cells. This method can readily be used to search for EGFR modulators in both chemical and RNAi screening; with potential applicability to other receptor tyrosine kinases.

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Irksome

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