Titlebook: Isotope labeling in Biomolecular NMR; Hanudatta S. Atreya Book 2012 Springer Science+Business Media Dordrecht 2012 Biomolecular NMR.Geneti

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原告

G. A. Nagana Gowda,Narasimhamurthy Shanaiah,Daniel Raftery

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Mammalian Expression of Isotopically Labeled Proteins for NMR Spectroscopyction of isotopically enriched proteins. This system allows for the expression of mammalian proteins and their complexes, including proteins that require post-translational modifications. We describe how this system can produce isotopically labeled .N and .C post-translationally modified proteins, s

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Isotope Labeling Methods for Large Systems molecules. However, in the last two decades the size limit has been pushed upwards considerably and it is now possible to use NMR spectroscopy for structure calculations of proteins of molecular weights approaching 100 kDa and to probe dynamics for supramolecular complexes of molecular weights in e

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Segmental Labeling to Study Multidomain Proteinsexes. Segmental labeling is used to specifically label a segment of protein structure with NMR active nuclei, thus reducing NMR spectral complexity and greatly facilitating structural NMR studies of large multi-domain proteins. It can also be used to introduce a synthetic fragment into a protein str

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Isotope Labeling for Solution and Solid-State NMR Spectroscopy of Membrane Proteins with emphasis on integral membrane proteins (IMPs). While solution NMR is used to study IMPs under fast tumbling conditions, such as in the presence of detergent micelles or isotropic bicelles, solid-state NMR is used to study the structure and orientation of IMPs in lipid vesicles and bilayers. In