Titlebook: Introduction to Fluorescence Sensing; Alexander P. Demchenko Book 20152nd edition Springer International Publishing Switzerland 2015 cell

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Alexander P. Demchenkoes. This conflict derived from the process of political democratization that began in the 1990s and accelerated since 2001. There are two major factors responsible for this advance in democratization. First are the rapid socio-economic changes that have occurred since the latter half of the 1980s. E

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Basic Principles, interactions between molecules, particles and interfaces was suggested and tested for application in sensing. In this Chapter we provide a short survey of basic strategies in molecular sensing and try to determine the role of those of them that are based on fluorescence detection. Some of them are

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Theoretical Aspects,n every sensor, such as selectivity, sensitivity and limit of detection. Concentrating on reversible binding, in which the mass action law is observed, the methods of determining the binding constants and modeling the ligand binding isotherms are presented. Kinetics of target binding and its influen

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Molecular-Size Fluorescence Emitters,ponse units into sensing and imaging technologies and we discuss their general properties that are essential for their applications. Organic dyes were the first and remain to be the most popular emitters used with these purposes. Their labeling and responsive properties are overviewed. Natural fluor

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Nanoscale Fluorescence Emitters,n their applications in sensing and imaging technologies. These new materials are diverse. Strongly emissive nanoparticles can be formed of organic or inorganic polymers incorporating organic dyes. In dendrimers they can be attached covalently in the desired sites. Collective excitonic effects appea

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Fluorescent Nanocomposites,alone. These larger nanocomposites demonstrate dramatically increased brightness, the ability to concentrate emission on particular luminophores (antenna effect), shifting the emission spectrum to a desired extent (wavelength conversion), modulating emission anisotropy and lifetime. Such collective

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Mechanisms of Signal Transduction,its providing the response signal (.). Here we focus on the coupling between these functionalities. Such coupling must involve signal transduction resulting in changes on target biding of the reporter emission. The coupling mechanisms can be based on the excited-state reactions, such as electron tra