Titlebook: Intrinsically Disordered Proteins; Methods and Protocol Birthe B. Kragelund,Karen Skriver Book 2020 Springer Science+Business Media, LLC, p

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Exploring Protein Intrinsic Disorder with MobiDBically disordered. MobiDB is the main repository of protein disorder and mobility annotations, combining different data sources to provide an exhaustive overview of intrinsic disorder. MobiDB includes curated annotations from other databases, indirect disorder evidence from structural data, and diso

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Production of Intrinsically Disordered Proteins for Biophysical Studies: Tips and Tricksconsiderable effort has been put into the structural characterization of their conformational ensembles, to understand the link between the transient, short- and long-range organizations of IDPs and their functions. Such biophysical studies require substantial amounts of pure protein, representing a

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Recombinant Production of Monomeric Isotope-Enriched Aggregation-Prone Peptides: Polyglutamine Tractome low-complexity regions (LCRs) and short linear motifs (SLiMs). In particular, polyglutamine (polyQ) tracts are LCRs of variable length highly enriched in glutamine residues. They are common in transcription factors, and their length can have an impact on transcriptional activity. In nine protein

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Cell-Free Protein Synthesis of Small Intrinsically Disordered Proteins for NMR Spectroscopyse of CFPS has almost from the onset been favorably linked to the production of isotopically labelled proteins for NMR spectroscopy as the resulting labelling of the produced protein is defined by the chosen amino acids during reaction setup. Here we describe how to set up production and isotopic la

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Quantitative Protein Disorder Assessment Using NMR Chemical Shifts and pluripotency of intrinsically disordered proteins (IDPs). The first step toward a better understanding of IDPs is a quantitative and position-specific experimental characterization, and nuclear magnetic resonance (NMR) spectroscopy has emerged as the method of first choice. Here, we describe ho