Titlebook: Intracellular Lipid Transport; Methods and Protocol Guillaume Drin Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Following Anterograde Transport of Phosphatidylserine in Yeast in Real Time,opy using the specific PS reporter C2.-GFP. We describe the construction of the required . yeast strain and the preparation of lyso-PS. We present an example of the use of this assay to follow the activity of the yeast PS transport proteins Osh6 and Osh7.

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Monitoring and Modulating Intracellular Cholesterol Trafficking Using ALOD4, a Cholesterol-Binding this question. ALOD4 traps cholesterol at the PM, leading to depletion of ER cholesterol without altering total cell cholesterol. The effects of ALOD4 are reversible. This tool has been used to show that the ER is able to continuously sample cholesterol from PM, providing ER with information about levels of PM cholesterol.

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