Titlebook: Intestinal Stem Cells; Methods and Protocol Paloma Ordó?ez-Morán Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 20

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Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategyelial organoids generated from patient-derived tissues, from induced pluripotent stem cell (iPSC) derived intestinal organoids, and from freshly isolated patient tissue intestinal crypts. These methods will facilitate experimental analysis of human LGR5-expressing cells in development, wound healing, and cancer.

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Autophagy Detection in Intestinal Stem Cellsng autophagosomes in GFP-LC3 transgenic mice and quantifying the p62 protein levels. We also describe methods for detecting mitophagy in these cells, by analyzing the mitochondrial transmembrane potential and reactive oxygen species (ROS) level by MitoTracker and CellROX solution, respectively.

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