Titlebook: Interferon Methods and Protocols; Daniel J. J. Carr Book 2005 Humana Press 2005 cancer.gene therapy.infection.interferon.research.resistan

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Genomic DNA Affinity Chromatography,of specific transcription factors, which then translocate into the nucleus to bind to cognate gene elements in the promoters of IFN-inducible genes. The prototypic IFN-inducible transcription factors are the signal transducer and activator of transcription (STAT) proteins. IFN-receptor interactions

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,A Convenient and Sensitive Fluorescence Resonance Energy Transfer Assay for RNase L and 2′,5′ Oligolated, 2′,5′-linked oligoadenylates (2–5A) are produced in mammalian cells by interferon-inducible 2–5A synthetases (OAS) in response to viral double-stranded RNA. 2–5A activates a uniquely regulated endoribonuclease, RNase L, resulting in the cleavage of single-stranded viral and cellular RNAs, thu

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Mitogen-Activated Protein Kinase Pathways in Interferon Signaling,ype I interferon signaling and the generation of interferon responses. In particular, the p38 MAP kinase cascade exerts positive regulatory roles on transciptional activation of interferon-sensitive genes (ISGs) and the induction of the antiproliferative and antiviral properties of interferons. In t

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Development of an Interferon-Based Cancer Vaccine Protocol,feron (IFN)-α to create cancer vaccine cells. This protocol has been used to develop cancer vaccines in mice against B16 melanoma, RM-1 prostate cancer, and P388 lymphocytic leukemia. A detailed description of the protocol is presented. Important considerations that are discussed include the method

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Flow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed Populations, in response to viral infection and other stimuli. PDCs are a rare cell type, making up less than 0.5% of peripheral blood mononuclear cells. To date, these cells have not been successfully cultured in vitro and are very sensitive to selection via magnetic bead labeling, making them very difficult t

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