Titlebook: Insoluble Proteins; Methods and Protocol Elena García-Fruitós Book 2015 Springer Science+Business Media New York 2015 Biotechnology.Express

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Co-translational Stabilization of Insoluble Proteins in Cell-Free Expression Systemsccessibility of cell-free reactions allows addressing such critical steps by the addition of protein stabilizers such as chemical chaperones or detergents directly into the expression reactions. This approach could therefore reduce or even prevent initial protein precipitation already in the transla

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Functional Expression of Plant Membrane Proteins in ,c bacterium, has been traditionally used in food fermentations and is nowadays widely used in biotechnology for large-scale production of heterologous proteins. This system has been successfully used for the production of prokaryotic and eukaryotic membrane proteins. The purpose of this chapter is t

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Recombinant Glycoprotein Production in Human Cell Linesified by a large number of posttranslational modifications. These modifications can occur during or after the synthesis process, and glycosylation appears as the most common posttranslational modification. It is estimated that 50 % of human proteins have some kind of glycosylation, which has a key r

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Ranjana Arya,Jamal S. M. Sabir,Roop S. Bora,Kulvinder S. Sainiation advice from the expertsThis volume provides current methods to analyze the properties of designer receptors exclusively activated by designer drugs (DREADDs) in vitro and to measure the biological responses of DREADD activation in different neuronal populations in vivo. Chapters focus on the u