Titlebook: Inositol Phosphates; Methods and Protocol Gregory J. Miller Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Di

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as a Model to Study Inositol Polyphosphates and Inorganic Polyphosphatege of the metabolic as well as functional connections between inositol polyphosphates and inorganic polyphosphate, we have refined and developed techniques to extract and analyze these molecules in a second eukaryotic experimental model, the amoeba .. This amoeba, possessing a well-defined developme

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Characterizing Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Familysubstrates, including diphosphoinositol polyphosphates (inositol diphosphates, inositol pyrophosphates), dinucleotide phosphates, nucleosides as well as 5-phosphoribosyl 1-pyrophosphate and inorganic polyphosphate. Here, we describe protocols to optimize these enzymes, with consideration to buffer c

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Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells-mediated cellular responses. Biochemical and genetic studies have revealed diverse roles of IP and PI signaling in eukaryotic signaling, but detailed characterization of unique IP and PI signaling profiles in response to different agonists and among cell types remains largely unexplored. Here, we o

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