Titlebook: Innate Immune Activation; Methods and Protocol Dominic De Nardo,Christine M De Nardo Book 2018 Springer Science+Business Media LLC 2018 NFB

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Bioinformatic Assessment of Macrophage Activation by the Innate Immune System,f CD14. monocytes into macrophages followed by in vitro stimulations to identify stimulation-specific gene modules. These gene modules can be used for experimental validation, as well as further bioinformatic analysis to determine key pathways or upstream transcription factors.

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Generation of Innate Immune Reporter Cells Using Retroviral Transduction, of retroviruses, retroviral infection of innate immune target cells (including isolation and differentiation of murine bone marrow cells to macrophages), and several methods for enrichment of positively transduced cells.

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Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis,The inter-domain cleavage of Gasdermin-D is a reliable marker for the activation of inflammatory caspases and cell pyroptosis. Here, we describe the methods for examining Gasdermin-D cleavage by activated inflammatory caspases in vitro and upon inflammasome activation in vivo.

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Olaf-Oliver Wolz,Manfred Koegl,Alexander N. R. Weberg evolutionary algorithms to data mining is that evolutionary algorithms are robust search methods which perform a global search in the space of candidate solutions (rules or another form of kn978-3-642-07763-0978-3-662-04923-5Series ISSN 1619-7127 Series E-ISSN 2627-6461

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Roland Felix Dreier,José Carlos Santos,Petr Broz Ph.D.