Titlebook: Inflammation and Cancer; Methods and Protocol Brendan J. Jenkins Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), u

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An In Vitro Model for Assessing Acute Lung Injury During Pancreatitis Development Using Primary Mousng this complication are ill-defined. In recent years, in vitro co-culture systems have emerged as powerful tools for studying complex interactions between different cell types in disease. In the context of pancreatitis, pancreatic acinar epithelial cells produce and secrete digestive enzymes, and t

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Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation, and the efficient resolution of inflammation that minimizes damage to the host. The peritoneal cavity provides an accessible and physiologically relevant system where the delicate balance of these processes may be studied. Here, we describe murine models of peritoneal inflammation that enable stud

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Tracking Cardiovascular Comorbidity in Models of Chronic Inflammatory Diseasen cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory

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Flow Cytometry Identification of Cell Compartments in the Murine Brainodendroglioma and astrocytoma) are thought to arise from neuronal and glial progenitor populations in the brain and are poorly infiltrated by immune cells. An improved understanding of oligodendrocytes, astrocytes, and the immune environment throughout tumor development will enhance the analysis and

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Expression and Purification of Inflammasome Sensor NOD-Like Receptor Protein-1 Using the Baculovirusnent of inflammasomes is the sensor protein, which, upon sensing pathogen- or danger-associated molecular patterns (PAMPs or DAMPs), converts from inactive to active signaling platform for initiation of inflammatory signaling. A reliable source for the production and purification of recombinant infl