Titlebook: Inflammation; Methods and Protocol Bj?rn E. Clausen,Jon D. Laman Book 2017 Springer Science+Business Media LLC 2017 human IMID.characteriza

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Hapten-Specific T Cell-Mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammator should allow to determinate their specific functions in the CHS model. Here, we describe procedures to extract those cells from the skin and to analyze them by flow cytometry using a combination of appropriate surface markers allowing further transcriptomic analysis and functional assays.

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Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Models, epidermal thickening, and scaling that jointly greatly resembles human psoriasis. Here we describe the use of surgical denervation in the imiquimod-induced psoriasiform model, to study the role of skin innervation and neuropeptides in the pathogenesis of psoriasis.

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Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodesand quantity of RNA that often limits analyses of complex mixtures of immune cell populations. Here, we describe a protocol to isolate rare T cell populations from the lung and in particular the subsequent methods to isolate high-grade RNA in order to perform NGS-based transcriptome analyses.

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1064-3745 ation advice from the experts.Includes supplementary materiaThis volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. .Inflammation: Methods and Protocols .is divided into four sections: the f

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Induction and Analysis of Bronchus-Associated Lymphoid Tissueuces highly organized BALT but distinct pathways and molecules are used to promote the development of BALT. Here, we describe the induction and phenotype of the distinct types of BALT as well as the immunofluorescence microscopy-based analysis of the induced lymphoid tissue in the lung.

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Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mous physiologically relevant house dust mite (HDM) allergen, which parallel eosinophilic airway inflammation observed in asthma patients. Here, we describe the analysis of ILC2 by flow cytometry in these two commonly used allergic airway inflammation models in the mouse.

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