Titlebook: In Vitro Mutagenesis Protocols; Jeff Braman Book 20022nd edition Humana Press 2002

隱語

Neonatal

Analogy

Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids,xtensively explored, as illustrated by several chapters in this volume. In particular, the relative ease with which DNA fragments of 2–3 kb can be generated facilitated the development of the highly efficient procedure known as “recombination PCR” (RPCR), the principles of which were first described

Lipoma

嚙齒動物

吵鬧

Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA,have been described, these methods include such drawbacks as the possibility of generating undesired secondary mutations, because of the low fidelity of some of the thermostable DNA polymerases, the need for four or more specific oligonucleotide primers, and the use of sophisticated, individually op

轉(zhuǎn)換

偽善

prosthesis

Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction, a given protein of interest. In this, site-directed mutagenesis has proven to be a valuable technique. After the advent of polymerase chain reaction . mediated mutagenesis has been the method of choice to change from one amino acid to another, which has replaced the classic Kunkel’s method (.).

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