Titlebook: In Vitro Mutagenesis Protocols; Michael K. Trower Book 19961st edition Humana Press 1996

Facilities

Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template,study of structure-function relationships or modification of restriction endonuclease restriction sites. Several novel polymerase chain reaction (PCR)-based methods of in vitro mutagenesis have been described (.–.). Site-specific mutations are created by introducing mismatches into the oligonucleoti

VEST

無(wú)辜

寬宏大量

花束

Water-Brash

Nonthreatening

CLAM

interpose

Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template,so been widely used as a quick and efficient alternative for introducing mutations into specific DNA sequences. Various general methods for site-directed mutagenesis using the PCR have been described recently (.–.). In the overlap extension method two pairs of primers are used to generate two DNA fr

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Site-Directed Mutagenesis In Vitro by Megaprimer PCR,le. The method utilizes three oligonucleotide primers and two rounds of PCR performed on a DNA template containing the cloned gene to be mutated (.–.). The basic megaprimer procedure is shown schematically in ., where A and B represent the “flanking” primers that can map either within the cloned gen