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Titlebook: Immunocytochemical Methods and Protocols; Lorette C. Javois Book 19992nd edition Humana Press 1999 DNA.ELISA.Filtration.Organelle.PCR.cell

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書目名稱Immunocytochemical Methods and Protocols
編輯Lorette C. Javois
視頻videohttp://file.papertrans.cn/463/462124/462124.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Immunocytochemical Methods and Protocols;  Lorette C. Javois Book 19992nd edition Humana Press 1999 DNA.ELISA.Filtration.Organelle.PCR.cell
描述Lorette Javois‘ timely new 2nd edition revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization..
出版日期Book 19992nd edition
關(guān)鍵詞DNA; ELISA; Filtration; Organelle; PCR; cell membrane; chemistry; electron microscopy; hybridization; membran
版次2
doihttps://doi.org/10.1385/1592592139
isbn_ebook978-1-59259-213-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1999
The information of publication is updating

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Overview of Cell Fixatives and Cell Membrane Permeantsroteins and carbohydrates. This chapter will review some of the advantages and drawbacks of common chemical fixation and permeabilization methods used for immunolocalization at the level of light microscopy.
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Biotinylation of Antibodiespplied to the sample, and the detection is accomplished by using labeled avidin. Avidin with a variety of labels are available commercially, including fluorescent, enzyme, iodine, ferritin, or gold labels.
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Book 19992nd editioncroscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization..
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1064-3745 , light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization..978-1-59259-213-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Conjugation of Fluorochromes to Antibodiesed. The primary antigenic site is thus revealed. A major advantage of the indirect method is the enhanced sensitivity. In addition, a labeled secondary antibody can be used to locate any number of primary antibodies raised in the same animal species without the necessity of labeling each primary antibody.
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