Titlebook: Immune Homeostasis; Methods and Protocol Vijay Kumar Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Modulation and Determination of the Status of Inflammasomes in ,-Infected Macrophages,t for their survival. When the host encounters intracellular pathogens, a multimeric protein complex called NLRP3 inflammasome gets turned on, leading to caspase-1 activation-mediated maturation of IL-1β from its pro-form. However, . often manages to neutralize inflammasome activation by manipulatin

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Isolation and Culture of Mouse Primary Microglia and Oligodendrocyte Precursor Cells,of diseases, such as stroke, Alzheimer’s disease, multiple sclerosis, etc. Microglia and OPC serve as compelling tools for the study of CNS diseases as well as the repair and damage of myelin sheath in vitro. In this protocol, we summarized a method which is capable of using the same batch of new-bo

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exostosis

CRISPR-Cas9-Induced Gene Editing in Primary Human Monocytes,n widely used to manipulate specific genes of immortal monocyte cell lines to study monocyte functions. However, human primary monocytes are refractory to this method with low gene knockout (KO) efficiency. In this chapter, we developed an in vitro gene-editing procedure for primary human monocytes

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Rheumatologist

https://doi.org/10.1007/978-1-0716-3754-8Regulated immune network; Cancer; Neurodegenerative diseases; Infections; Autoimmune diseases